O SELECȚIE URIAȘĂ
Peste 4 milioane de cărți în engleză la prețuri avantajoase.
ISBN | 9780132700344 |
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Autor | Winfrey Michael |
Editura | Prentice Hall |
Tip copertă | Paperback |
Anul publicării | 1997 |
Număr de pagini | 400 |
This manual encompasses an integrated series of molecular biology laboratory exercises that involve the cloning and analysis of the bioluminescence (lux) genes from the marine bacterium Vibrio fischeri. KEY TOPICS: The manual is divided into discrete units with each demonstrating one or more aspects of the cloning project. The manual is based on one of nature's most fascinating biological phenomenon: the biological production of light. This results in a recurrent theme of interest and makes the project very relevant to interdisciplinary topics such as fish symbiosis, biochemistry, biophysics, etc. Includes instruction in the basic techniques of modern molecular biology: DNA isolation and analysis, DNA restriction, agarose gel electrophoresis, ligations, transformation of recombinant DNA, preparation and screening a genomic library, restriction mapping, Southern blotting, hybridization, DNA sequencing, pulsed field gel electrophoresis. MARKET: Designed for a one semester course in Molecular Biology. Also appropriate for a molecular biology component of Microbial Genetics, Genetics, Biochemistry, or Advanced Microbiology courses.