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Zymography

Name: Zymography
Brand: Springer, Berlin
SKU: 9781071644812
Price: 1190.26 RON
Availability: InStock

Biological and Clinical Applications, Volume 2

Autor: Raouf Khalil

Limbă:

Legare: Copertă tare

Editura: Springer, Berlin

Disponibilitate: În depozitul extern

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1 190.26 lei

Volume 2 describes how to determine the activity of different isozymes, allozymes, and families of p...

Informații despre carte

Autor

Raouf Khalil

Limbă

engleză

Legare

Carte - Copertă tare

Publicat

2025

Pagini

290

EAN

9781071644812

Enbook ID

47176696

Editura

Springer, Berlin

Greutate

687

Dimensiuni

155 x 235

Descriere completă

Volume 2 describes how to determine the activity of different isozymes, allozymes, and families of proteinases to advance the fields of enzymology and molecular evolution, and provides useful biomarkers for various biological processes, pathological conditions, and clinical disorders. The chapters in Volume 2 are organized in three parts. Part I introduces in situ zymography and localization of bright green-fluorescent gelatinase activity in tissue sections, in situ zymography in formalin-fixed paraffin-embedded and mineralized tissues, and in vivo zymography as an essential activity assay for studying the activity of matrix metalloproteinases (MMPs) in a cell-specific manner in the brain. Part II focuses on biological applications of zymography such as fundamentals of zymography and its applications to the study of biological samples, gelatin zymography to quantify MMP-2 and MMP-9 in complex biological specimens, and detection of proteolytic enzymes in polyacrylamide gels supplemented with diverse biological substrates. Part III focuses on potential clinical applications of zymography, with chapters describing assessment of MMP-2 and MMP-9 hydrolytic activity in preclinical and clinical tissue samples, the use of zymography to assess circulating MMP-2 and MMP-9 in plasma and serum and in pathological conditions, and the use of zymography for the detection of bacterial proteases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Zymography: Biological and Clinical Applications, Volume II is a valuable resource for both experts in the field, as well as new scientists aspiring to learn and perform successful zymography techniques.

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Zymography

Biological and Clinical Applications, Volume 2

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