ZebraTrack: An Automated Method to Study Zebrafish Behaviour

Name: ZebraTrack: An Automated Method to Study Zebrafish Behaviour
Brand: LAP Lambert Academic Publishing
SKU: 9783659900518
Price: 172.76 RON
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Autor: Yogesh Bhargava, Anamika Bhargava

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Editura: LAP Lambert Academic Publishing

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172.76 lei

Behavioural neuroscience relies on software driven methods for behavioural assessment. Therefore, we...

Informații despre carte

Autor

Yogesh Bhargava, Anamika Bhargava

Limbă

engleză

Legare

Carte - Carte broșată

Publicat

2017

Pagini

EAN

9783659900518

Enbook ID

16014008

Editura

LAP Lambert Academic Publishing

Greutate

107

Dimensiuni

150 x 220 x 4

Descriere completă

Behavioural neuroscience relies on software driven methods for behavioural assessment. Therefore, we propose a novel method which we called as ZebraTrack. It includes cost-effective imaging setup for distraction-free behavioural acquisition, automated tracking using open-source ImageJ software and workflow for extraction of behavioural endpoints. Our ImageJ algorithm is capable of providing control to users at key steps while maintaining automation in tracking without the need for the installation of external plugins. We have validated this method by testing novelty induced anxiety behaviour in adult zebrafish. Our results, in agreement with established findings, showed that during state-anxiety, zebrafish showed reduced distance travelled, increased thigmotaxis and freezing events. Furthermore, we proposed a method to represent both spatial and temporal distribution of choice-based behaviour which is currently not possible to represent using simple videograms. ZebraTrack method is simple and economical, yet robust enough to give results comparable with those obtained from costly proprietary software like Ethovision XT.

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ZebraTrack: An Automated Method to Study Zebrafish Behaviour

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