Winemaking from the vineyard to shipment of bottled product is a series of challenges for the winemaking staff. The introductory narrative is designed be an overview, from the wine microbiologist s point of view, of those critical junctures in the process (CCPs) that are of concern in wine quality as well as intervention/control programs to address them.The second edition of Wine Microbiology builds upon the foundation of its highly successful predecessor with emphasis on modern molecular methods.§Among changes and additions are: additional micrographs chapter on laboratory safety collection of laboratory methods expanded Appendices including basic microscopy and setup of the microbiology laboratory, identification of chemical instabilities often confused with bacteria, media selection and preparation, microbiological dyes and stains, aseptic technique, estimation of population density and, lastly, new technology for characterization/enumeration.