Carte Transgenic Xenopus Shlomo Seidman

Transgenic Xenopus

Microinjection Methods and Developmental Neurobiology

Limbă: engleză
Legare: Copertă tare
Disponibilitate: În depozitul extern
Expediem în 10-13 zile
550.27 lei
Two distinguished international experts offer a fully illustrated, thoroughly referenced guide to th...

Informații despre carte

Limbă
engleză
Legare
Carte - Copertă tare
Publicat
1996
Pagini
198
EAN
9780896034570
ISBN
0896034577
Enbook ID
01400731
Greutate
547
Dimensiuni
155 x 235 x 18

Descriere completă

Two distinguished international experts offer a fully illustrated, thoroughly referenced guide to the use of microinjected Xenopus embryos to study the role and spatiotemporal regulation of nervous system proteins during vertebrate development. Drs. Seidman and Soreq include all the basic scientific background and protocols needed to consider and exploit the vast potential of this model system. They provide an outline of Xenopus development, detailed methods for in vitro fertilization and microinjection, and protocols and references for a variety of biochemical and histochemical techniques to analyze the results of a microinjection experiment. They also present extensive experimental data illustrating the use of enzyme activity assays, gel electrophoresis, density ultracentrifugation, EAIA, RT-PCR, cytochemical and immunocytochemical staining, and electron microscopy. Transgenic Xenopus demonstrates convincingly the special utility of Xenopus embryo microinjection as a sophisticated and versatile tool for studying nervous system proteins.

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