Thin-Layer Chromatography with Flame Ionization Detection

Name: Thin-Layer Chromatography with Flame Ionization Detection
Brand: Springer
SKU: 9789401081597
Price: 276.07 RON
Availability: InStock

Autor: M. Ranny

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Editura: Springer

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Thin-layer chromatography (TLC) has become a common and much favoured separation technique in labora...

Informații despre carte

Autor

M. Ranny

Limbă

engleză

Legare

Carte - Carte broșată

Publicat

2011

Pagini

200

EAN

9789401081597

ISBN

940108159X

Enbook ID

02185373

Editura

Springer

Greutate

314

Dimensiuni

155 x 235 x 12

Descriere completă

Thin-layer chromatography (TLC) has become a common and much favoured separation technique in laboratories in widely varied fields in recent years. Much of the credit for the introduction of this technique into analytical practice at the l 2 end of the 1950s is due to E. Stahl This method is simple and is characterized by high separation ability and sufficient sensitivity3; however, some analysts feel that it has passed the peak in its development and will gradually be replaced by the more modem high-performance liquid chromatography (HPLC). This is undoubtedly a very important analytical technique utilizing the specific separa tion properties of a large number of sorbents and the possibility of regulating 4 the flow-rate of the mobile phase by adjusting the pressure Standardization of the experimental conditions is simpler in HPLC than in TLC, where the activity of the sorbent and flow-rate of the eitlent in the thin layer depend markedly on the relative humidity of the laboratory atmosphere and on the composition of the gaseous phase in the elution chamber. In addition, systems for quantitative detection of the separated ~ones are better developed for HPLC than for classical TLC, where, until recently, cumbersome and often even insufficiently reproducible chemical or gravimetric analysis of the extracts of scraped-off spots or densitometry of the separated zones, located first by pyrolysis or reaction s with suitable detection agents, were the predominant determination methods .

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Thin-Layer Chromatography with Flame Ionization Detection

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