This volume aims at providing an up-to-date§compilation of current methodological approaches utilized for the exploration§of nucleolar structure and function. The§Nucleolus: Methods and Protocols guides readers through imaging of the§nucleolus, analysis of ribosomal RNA transcription and processing, and genomics§and proteomics of the nucleolus. Written in the highly successful Methods in§Molecular Biology series format, chapters include introductions to their§respective topics, lists of the necessary materials and reagents, step-by-step,§readily reproducible laboratory protocols, and tips on troubleshooting and§avoiding known pitfalls.§§Authoritative and practical, The Nucleolus: Methods and Protocols provides a diversity protocols of the nucleolus§and provides scientists with a reliable practical handbook to facilitate the§investigation of this nuclear compartment at the advanced level.§§