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Carte CRAC Channel Aubin Penna

CRAC Channel

Methods and Protocols

Autor: Aubin Penna, Bruno Constantin
Limbă: engleză
Legare: Copertă tare
Editura: Humana Press Inc.
Disponibilitate: În depozitul extern
Expediem în 10-13 zile
538.67 lei
This volume discusses the cellular and molecular techniques used to study and characterize the diffe...

Informații despre carte

Autor
Aubin Penna, Bruno Constantin
Limbă
engleză
Legare
Carte - Copertă tare
Publicat
2018
Pagini
204
EAN
9781493987023
ISBN
149398702X
Enbook ID
19434761
Editura
Humana Press Inc.
Greutate
645
Dimensiuni
178 x 254 x 15

Descriere completă

This volume discusses the cellular and molecular techniques used to study and characterize the different components of the CRAC channel signaling pathway. The chapters in this book cover topics such as fluorescence-based measurements of the CRAC channel activity in cell population; patch-clamp recording of the CRAC channel in STIM-Orai overexpressing cells and native systems; western-blotting and co-immunoprecipitation of endogenous STIM/ORAI and protein partners; shRNA-mediated gene silencing; and the SC-SMD system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, The CRAC Channel: Methods and Protocols is a valuable resource for any researcher interested in learning more about the CRAC channel--its activation mechanism and roles in cellular functions still remains to be completely explored.

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