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Carte Plant Virology Protocols Gary D. Foster

Plant Virology Protocols

From Virus Isolation to Transgenic Resistance

Autor: Gary D. Foster, Sally Taylor
Limbă: engleză
Legare: Copertă tare
Editura: Humana Press Inc.
Disponibilitate: În depozitul extern în cantități mici
Expediem în 13-18 zile
890.44 lei
Plant Virology Protocols offers for the first time a comprehensive collection of state-of-the-art te...

Informații despre carte

Autor
Gary D. Foster, Sally Taylor
Limbă
engleză
Legare
Carte - Copertă tare
Publicat
1998
Pagini
571
EAN
9780896033856
ISBN
0896033856
Enbook ID
01400699
Editura
Humana Press Inc.
Greutate
1001
Dimensiuni
155 x 235 x 32

Descriere completă

Plant Virology Protocols offers for the first time a comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus. The authoritative contributors also discuss the history and mechanisms of coat protein-mediated protection and the key ethical issues surrounding transgenic technology. §Unprecedented in its comprehensiveness and its many time-proven methods, Plant Virology Protocols is certain to become the authoritative standard reference for plant scientists who want to tap the enormous benefits this technology has to offer.A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.

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