Plant Proteomics

Name: Plant Proteomics
Brand: Humana Press Inc.
SKU: 9781617376863
Price: 891.68 RON
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Methods and Protocols

Autor: Hervé Thiellement

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Legare: Carte broșată

Editura: Humana Press Inc.

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Plant Proteomics: Methods and Protocols provides up-to-date methods and protocols for plant proteomi...

Informații despre carte

Autor

Hervé Thiellement

Limbă

engleză

Legare

Carte - Carte broșată

Publicat

2010

Pagini

416

EAN

9781617376863

ISBN

1617376868

Enbook ID

01432268

Editura

Humana Press Inc.

Greutate

609

Dimensiuni

152 x 229 x 24

Descriere completă

Plant Proteomics: Methods and Protocols provides up-to-date methods and protocols for plant proteomics, each written by expert researchers from leading laboratories. Experimental methodologies are explained in clear and complete detail for both the wet and dry laboratories. The methods and protocols presented in this volume will inspire the novice as well as the expert researcher to undertake new experimental endeavors in plant proteomics.§Chapters 1 and 2 describe standard protocols and chapters 3 through 10 contain optimized protocols for protein extraction of tissues such as cereal seeds, xylem, phloem sap, of woody plants, and of cellular compartments, such as chloroplasts and mitochondria. These methods rely on improvements to solubilization of proteins, which is the emphasis of Chapters 11 and 12. Chapters 13 to 23 outline methods for protein identification, including 2DLC or LC of intact proteins, mass spectrometry (MALDI-TOF and MS/MS) with proteins separated by 2D gels, blue native ID gels, and Edman sequencing. Chapters 24 to 29 focus on the rapidly expanding area of analysis of post-translational modifications and protein-protein relationships. Attention is also given to data that proteomics produces, including management of that data, and to statistical tools used for effective analysis.

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Plant Proteomics

Methods and Protocols

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