Organelle Proteomics

Name: Organelle Proteomics
Brand: Humana Press Inc.
SKU: 9781617377723
Price: 576.66 RON
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Autor: Delphine Pflieger, Jean Rossier

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Editura: Humana Press Inc.

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Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identif...

Informații despre carte

Autor

Delphine Pflieger, Jean Rossier

Limbă

engleză

Legare

Carte - Carte broșată

Publicat

2010

Pagini

421

EAN

9781617377723

ISBN

1617377724

Enbook ID

01432349

Editura

Humana Press Inc.

Greutate

670

Dimensiuni

155 x 235 x 23

Descriere completă

Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study.This is the first book to examine organelle proteomics in depth.§It begins by introducing the different analytical strategies developed and successfully utilized to study organelle proteomes, and detailing the use of multidimensional liquid chromatography coupled to tandem mass spectrometry for peptide sample analysis. Detailed protocols are provided and a section is devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle.

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