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Carte Multimerization strategy to create an artificial pore-forming protein Fichera Claudia a

Multimerization strategy to create an artificial pore-forming protein

Autor: Fichera Claudia a, De Pinto Vito
Limbă: engleză
Legare: Carte broșată
Editura: Scholars' Press
Disponibilitate: În depozitul extern
Expediem în 5-8 zile
219.87 lei
Porines generate little or no selective water channels, sometimes voltage-dependent, and several of...

Informații despre carte

Autor
Fichera Claudia a, De Pinto Vito
Limbă
engleză
Legare
Carte - Carte broșată
Publicat
2014
Pagini
64
EAN
9783639663006
ISBN
3639663004
Enbook ID
09030219
Editura
Scholars' Press
Greutate
104
Dimensiuni
152 x 229 x 4

Descriere completă

Porines generate little or no selective water channels, sometimes voltage-dependent, and several of them play important roles in specific cellular pathways. Through a preliminary bioinformatic analysis was identified a pattern of basic protein with a hairpin structure ( -strand/loop/ -strand) from the PA83 protein of Bacillus anthracis. Through the realization of a very quick and simple experimental protocol we built an artificial construct with seven multimers of the basic module. The experimental plan developed allowed us to obtain in a few days a chimeric protein completely artificial, which was cloned into a bacterial expression vector. Expression experiments has carried out interesting results since we observed that the induced bacterial cultures were less cloudy, indicating a reduced cell growth. In accordance with the recent literature we are inclined to believe that the protein acts like antibiotic, fitting massively in the membrane and causing cell lysis of the host bacteria. This strategy could be used for the production of artificial proteins with biological, pharmaceutical and industrial high-impact.

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