Methods of Protein Microcharacterization

Name: Methods of Protein Microcharacterization
Brand: Humana Press Inc.
SKU: 9780896030909
Price: 551.47 RON
Availability: InStock

A Practical Handbook

Autor: John E. Shively

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Legare: Copertă tare

Editura: Humana Press Inc.

Disponibilitate: În depozitul extern

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Milestones in the techniques and methodology of polypeptide structure determination include the dete...

Informații despre carte

Autor

John E. Shively

Limbă

engleză

Legare

Carte - Copertă tare

Pagini

456

EAN

9780896030909

ISBN

0896030903

Enbook ID

01400556

Editura

Humana Press Inc.

Greutate

1920

Dimensiuni

155 x 235 x 30

Descriere completă

Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach by Laursen in 1971 (4) and the use of modified Edman reagents such as 4-N,N-dimethylaminoazobenzene-4'-isothiocy- ate (DABITC) for manual sequencing by Chang et al. (5) in 1976. A second major breakthrough in the analysis of polypeptides was automated amino acid analysis described by Spackman et al. in 1958 (6). However, during the period from 1975 to 1980, it became increasingly clear that the amount of material required for struc tural analysis was more than could be easily isolated for the vast majority of proteins. The field was criticized for its lack of sensitive techniques for the analysis of growth factors, immune modulators, membrane receptors, and peptide hormones. In addition, very little had been done to modernize and improve the original instruments introduced in the mid-1960s. The first indications of improved instrumentation for Edman chemistry came from Wittmann-Liebold's laboratory (7), followed by the introduction of a "micro" sequencer by Hunkapiller and Hood in 1978 (8). The movement toward improved instrumentation culminated in the "gas"-phase sequencer of Hewick et al. (9) in 1981.

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Methods of Protein Microcharacterization

A Practical Handbook

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Descriere completă

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