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This volume expands upon the previous edition with current, detailed protocols for investigating membranes and their component lipids in artificial membranes, cells, and in silico . Chapters focus on properties of the component lipids, membranes and their biophysical properties, fluorescent probes for studying membranes, sample preparation, physical techniques to study membrane composition, properties , and function, behavior of cholesterol within a bilayer and examination of cholesterol-dependent phase separation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.§§Authoritative and practical, Methods in Membrane Lipids, Second Edition seeks to aid scientist in further study into membrane lipids.§
