Methods in Biological Oxidative Stress

Name: Methods in Biological Oxidative Stress
Brand: Humana Press Inc.
SKU: 9781617372001
Price: 580.65 RON
Availability: InStock

Autor: Kenneth Hensley, Robert A. Floyd

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Legare: Carte broșată

Editura: Humana Press Inc.

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580.65 lei

Free radicals and reactive oxidizing agents are now recognized as causes or contributing factors in...

Informații despre carte

Autor

Kenneth Hensley, Robert A. Floyd

Limbă

engleză

Legare

Carte - Carte broșată

Publicat

2010

Pagini

216

EAN

9781617372001

ISBN

1617372005

Enbook ID

01431912

Editura

Humana Press Inc.

Greutate

347

Dimensiuni

152 x 229 x 13

Descriere completă

Free radicals and reactive oxidizing agents are now recognized as causes or contributing factors in inflammation and other disease states and have become a major focus of interest in pharmacology, toxicology, physiology, and biochemistry. In Methods in Biological Oxidative Stress, expert researchers detail their best experimental methods for detecting free radicals and reactive oxygen species or their byproducts. The techniques range from established standard protocols to advanced methodologies using HPLC, mass spectrometry, and electron paramagnetic resonance. The HPLC techniques are applied to the electrochemical measurement of protein oxidation products, particularly nitrotyrosine and dityrosine, and to the electrochemical detection of DNA oxidation products. There are also mass spectrometry methods for measuring lipid oxidation products. Each technique is described in step-by-step detail to ensure robust results, with many presented by the experimenters who have led their development. Useful notes explain the pitfalls to avoid and the chemical principles underlying each method. §Authoritative and highly practical, Methods in Biological Oxidative Stress provides laboratory researchers and technicians a unique collection of standard, easy-to-use experimental methods for oxidative stress bioanalysis, plus cutting-edge instrumental techniques using HPLC, mass spectrometry, and EPR spectrometry.A collection of readily reproducible experimental methods for detecting free radicals and reactive oxygen species or their byproducts. The techniques range from established routine protocols to advanced methodologies using HPLC, mass spectrometry, and electron paramagnetic resonance. The HPLC techniques are applied to the electrochemical measurement of protein oxidation products, particularly nitrotyrosine and dityrosine, to the electrochemical detection of DNA oxidation products. There are also mass spectrometry methods measuring lipid oxidation products.

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