Methods in Biological Oxidative Stress

Name: Methods in Biological Oxidative Stress
Brand: Humana Press Inc.
SKU: 9780896038158
Price: 580.45 RON
Availability: InStock

Autor: Kenneth Hensley, Robert A. Floyd

Limbă:

Legare: Copertă tare

Editura: Humana Press Inc.

Disponibilitate: În depozitul extern în cantități mici

Expediem în 13-18 zile

580.45 lei

A collection of readily reproducible experimental methods for detecting free radicals and reactive o...

Informații despre carte

Autor

Kenneth Hensley, Robert A. Floyd

Limbă

engleză

Legare

Carte - Copertă tare

Publicat

2003

Pagini

216

EAN

9780896038158

ISBN

0896038157

Enbook ID

01400856

Editura

Humana Press Inc.

Greutate

504

Dimensiuni

155 x 235 x 23

Descriere completă

A collection of readily reproducible experimental methods for detecting free radicals and reactive oxygen species or their byproducts. The techniques range from established routine protocols to advanced methodologies using HPLC, mass spectrometry, and electron paramagnetic resonance. The HPLC techniques are applied to the electrochemical measurement of protein oxidation products, particularly nitrotyrosine and dityrosine, to the electrochemical detection of DNA oxidation products. There are also mass spectrometry methods measuring lipid oxidation products.Free radicals and reactive oxidizing agents are now recognized as causes or contributing factors in inflammation and other disease states and have become a major focus of interest in pharmacology, toxicology, physiology, and biochemistry. In Methods in Biological Oxidative Stress, expert researchers detail their best experimental methods for detecting free radicals and reactive oxygen species or their byproducts. The techniques range from established standard protocols to advanced methodologies using HPLC, mass spectrometry, and electron paramagnetic resonance. The HPLC techniques are applied to the electrochemical measurement of protein oxidation products, particularly nitrotyrosine and dityrosine, and to the electrochemical detection of DNA oxidation products. There are also mass spectrometry methods for measuring lipid oxidation products. Each technique is described in step-by-step detail to ensure robust results, with many presented by the experimenters who have led their development. Useful notes explain the pitfalls to avoid and the chemical principles underlying each method. §Authoritative and highly practical, Methods in Biological Oxidative Stress provides laboratory researchers and technicians a unique collection of standard, easy-to-use experimental methods for oxidative stress bioanalysis, plus cutting-edge instrumental techniques using HPLC, mass spectrometry, and EPR spectrometry.

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