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Carte Ion Channels James D. Stockand

Ion Channels

Methods and Protocols

Autor: James D. Stockand, Mark S. Shapiro
Limbă: engleză
Legare: Copertă tare
Editura: Humana Press Inc.
Disponibilitate: În depozitul extern
Expediem în 10-13 zile
826.62 lei
The diverse applications in this volume range from the study of allosteric regulation of ion channel...

Informații despre carte

Autor
James D. Stockand, Mark S. Shapiro
Limbă
engleză
Legare
Carte - Copertă tare
Publicat
2006
Pagini
216
EAN
9781588295767
ISBN
1588295761
Enbook ID
01430513
Editura
Humana Press Inc.
Greutate
554
Dimensiuni
152 x 229 x 22

Descriere completă

The diverse applications in this volume range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, and for studying channel regulation.It is now clear that ion channels play essential roles in cell biology and physiology and that their dysfunction is the root cause of many human diseases. In Ion Channels: Methods and Protocols, internationally recognized biomedical scientists describe recent technological breakthroughs and demonstrate their use in successful experimental designs. The diverse applications range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, for studying channel regulation and physiological function, and for genetic screening and investigating channelopathies. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.§Comprehensive and highly practical, Ion Channels: Methods and Protocols offer both novice and experienced researchers a versatile collection of proven techniques for illuminating the structure, function, and regulation of ion channel proteins and finding suitable drug targets when they cause disease.

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