Generation of cDNA Libraries

Name: Generation of cDNA Libraries
Brand: Humana Press Inc.
SKU: 9781617373336
Price: 783.72 RON
Availability: InStock

Methods and Protocols

Autor: Shao-Yao Ying

Limbă:

Legare: Carte broșată

Editura: Humana Press Inc.

Disponibilitate: În depozitul extern

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Since its invention and subsequent development nearly 20 years ago, po- merase chain reaction (PCR)...

Informații despre carte

Autor

Shao-Yao Ying

Limbă

engleză

Legare

Carte - Carte broșată

Publicat

2010

Pagini

338

EAN

9781617373336

ISBN

1617373338

Enbook ID

02717288

Editura

Humana Press Inc.

Greutate

657

Dimensiuni

152 x 254 x 19

Descriere completă

Since its invention and subsequent development nearly 20 years ago, po- merase chain reaction (PCR) has been extensively utilized to identify numerous gene probes in vitro and in vivo. However, attempts to generate complete and full-length complementary cDNA libraries were, for the most part, fruitless and remained elusive until the last decade, when simple and rapid methods were developed. With current decoding and potential application of human genome information to genechips, there are urgent needs for identification of functional significance of these decoded gene sequences. Inherent in bringing these app- cations to fruition is the need to generate a complete and full-length cDNA library for potential functional assays of specific gene sequences. Generation of cDNA Libraries: Methods and Protocols serves as a laboratory manual on the evolution of generation of cDNA libraries, covering both ba- ground information and step-by-step practical laboratory recipes for which p- tocols, reagents, operational tips, instrumentation, and other requirements are detailed. The first chapter of the book is an overview of the basics of generating cDNA libraries, which include the following: (a) the definition of a cDNA library, (b) different kinds of cDNA libraries, (c) differences between methods for cDNA library generation using conventional approaches and novel stra- gies, including reverse generation of RNA repertoires from cDNA libraries, and (d) the quality of cDNA libraries.

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Generation of cDNA Libraries

Methods and Protocols

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Descriere completă

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