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Gene Knockout Protocols

Name: Gene Knockout Protocols
Brand: Humana Press Inc.
SKU: 9781617370809
Price: 553.26 RON
Availability: InStock

Autor: Martin J. Tymms, Ismail Kola

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Legare: Carte broșată

Editura: Humana Press Inc.

Disponibilitate: În depozitul extern

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553.26 lei

Highly skilled investigators with extensive experience in gene targeting and mouse genetics describe...

Informații despre carte

Autor

Martin J. Tymms, Ismail Kola

Limbă

engleză

Legare

Carte - Carte broșată

Publicat

2010

Pagini

431

EAN

9781617370809

ISBN

1617370800

Enbook ID

01431866

Editura

Humana Press Inc.

Greutate

716

Dimensiuni

152 x 24 x 24

Descriere completă

Highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for the analysis of the mouse phenotype. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today.As the major task of sequencing the human genome nears completion and the full complement of human genes are catalogued, the task of understanding the normal biological functions of genes and how their alteration leads to diseased states becomes more imperative. In Gene Knockout Protocols, highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for genetic phenotype analysis. These proven methods contain step-by-step instructions, as well as notes on pitfalls to avoid, and emphasize techniques that are relevant to researchers carrying out gene targeting work. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. Issues such as the use of existing mouse mutation resources and the influence of genetic background and epigenetic effects upon phenotype are also covered.§State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today.

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