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Enzyme Stabilization and Immobilization

Methods and Protocols

Autor: Shelley D. Minteer

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Legare: Carte broșată

Editura: Humana Press Inc.

Disponibilitate: În depozitul extern

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This volume introduces the reader to the field of enzyme stabilization and the different theories of...

Informații despre carte

Autor

Shelley D. Minteer

Limbă

engleză

Legare

Carte - Carte broșată

Publicat

2018

Pagini

234

EAN

9781493982196

Enbook ID

20091879

Editura

Humana Press Inc.

Greutate

470

Dimensiuni

178 x 254 x 14

Descriere completă

This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives. The second part of the book discusses protocols for enzyme stabilization during enzyme immobilization, including common techniques like sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Protocols for a variety of enzymes are shown, but the enzymes are chosen as examples to show that these protocols can be used for both enzymes of biological importance, as well as enzymes of industrial importance. The final part details spectroscopic protocols, methods, and assays for studying the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Enzyme Stabilization and Immobilization: Methods and Protocols, Second Edition provides molecular biologists, biochemists, and biomedical and biochemical engineers with the state-of-the-art technical information required to effectively stabilize their enzyme of interest in a variety of environments (i.e., harsh temperature, pH, or solvent conditions).

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Enzyme Stabilization and Immobilization

Methods and Protocols

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