Carte Cytochrome P450 Protocols Ian R. Phillips

Cytochrome P450 Protocols

Limbă: engleză
Legare: Copertă tare
Disponibilitate: În depozitul extern în cantități mici
Expediem în 13-18 zile
892.84 lei
For this second edition of their much praised Cytochrome P450, the editors have collected accounts o...

Informații despre carte

Limbă
engleză
Legare
Carte - Copertă tare
Publicat
2005
Pagini
364
EAN
9781588294418
ISBN
1588294412
Enbook ID
01430426
Greutate
714
Dimensiuni
162 x 240 x 28

Descriere completă

For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.

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