Cryotechniques in Biological Electron Microscopy

Name: Cryotechniques in Biological Electron Microscopy
Brand: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
SKU: 9783642728174
Price: 523.48 RON
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Autor: Rudolf A. Steinbrecht, Karl Zierold

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Editura: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG

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To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-a...

Informații despre carte

Autor

Rudolf A. Steinbrecht, Karl Zierold

Limbă

engleză

Legare

Carte - Carte broșată

Publicat

2011

Pagini

297

EAN

9783642728174

ISBN

9783642728174

Enbook ID

06621049

Editura

Springer-Verlag Berlin and Heidelberg GmbH & Co. KG

Greutate

548

Dimensiuni

170 x 244 x 18

Descriere completă

To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.

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