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Cardiovascular Proteomics

Name: Cardiovascular Proteomics
Brand: Humana Press Inc.
SKU: 9781617376276
Price: 551.82 RON
Availability: InStock

Methods and Protocols

Autor: Fernando Vivanco

Limbă:

Legare: Carte broșată

Editura: Humana Press Inc.

Disponibilitate: În depozitul extern

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551.82 lei

Cardiovascular Proteomics: Methods and Protocols presents cutting-edge protocols and strategies for...

Informații despre carte

Autor

Fernando Vivanco

Limbă

engleză

Legare

Carte - Carte broșată

Publicat

2010

Pagini

432

EAN

9781617376276

ISBN

1617376272

Enbook ID

01432220

Editura

Humana Press Inc.

Greutate

628

Dimensiuni

152 x 229 x 24

Descriere completă

Cardiovascular Proteomics: Methods and Protocols presents cutting-edge protocols and strategies for proteomic evaluation of cardiovascular disease written by pioneering researchers in the field. Topics explored in this comprehensive volume include obtaining specific heart proteins, cutting-edge techniques for identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, and phage display techniques for deciphering the molecular diversity of blood vessels. Detailed protocols for the isolation of short- and long-term culture of the adult mouse cardiac myocytes are also included.§Numerous advanced proteomic techniques are addressed, including protein separation by two-dimensional electrophoresis and two-dimensional difference gel electrophoresis, liquid chromatography coupled to mass spectrometry, and the SELDI-TOF approach to searching for biomarkers of stroke in human serum or of hypertension in the serum of animal models. Proteomic strategies and protocols for studying the proteome of endothelial, arterial smooth muscle cells, foam cells, and circulating blood monocytes constitute a major element of this text. Cutting-edge techniques for the analysis of subproteomes from isolation to final characterization and the characterization of posttranslational modifications are also addressed. This useful snapshot of current proteomic techniques is a single source for protocols for the identification and validation of novel biomarkers and targets in cardiovascular diseases.This cutting-edge book presents protocols and strategies for proteomic evaluation of cardiovascular disease written by pioneering researchers in the field. Topics explored in this comprehensive volume include obtaining specific heart proteins, techniques for identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, and phage display techniques for deciphering the molecular diversity of blood vessels.