Baculovirus and Insect Cell Expression Protocols

Autor: DAVID W. MURHAMMER

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Editura: Humana Press Inc.

Disponibilitate: În depozitul extern

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700.64 lei

The third edition of this volume expands upon the previous two editions with new and up-to-date meth...

Informații despre carte

Autor

DAVID W. MURHAMMER

Limbă

engleză

Legare

Carte - Carte broșată

Publicat

2018

Pagini

508

EAN

9781493979783

ISBN

9781493979783

Enbook ID

19304584

Editura

Humana Press Inc.

Greutate

940

Dimensiuni

178 x 254 x 36

Descriere completă

The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.

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