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Carte Bacterial Protein Secretion Systems Laure Journet

Bacterial Protein Secretion Systems

Autor: Laure Journet, Eric Cascales
Limbă: engleză
Legare: Copertă tare
Editura: Humana Press Inc.
Disponibilitate: În depozitul extern
Expediem în 10-13 zile
1 240.33 lei
This volume details protocols that cover the broad arsenal of techniques used to study a secretion s...

Informații despre carte

Autor
Laure Journet, Eric Cascales
Limbă
engleză
Legare
Carte - Copertă tare
Publicat
2017
Pagini
528
EAN
9781493970315
ISBN
1493970313
Enbook ID
15975773
Editura
Humana Press Inc.
Greutate
1216
Dimensiuni
178 x 254 x 35

Descriere completă

This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.

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