Assay Development for Lysyl Hydroxylase

Name: Assay Development for Lysyl Hydroxylase
Brand: VDM Verlag Dr. Müller
SKU: 9783639222579
Price: 253.91 RON
Availability: InStock

A new HPLC method for efficient separation of lysine- from hydroxylysine-containing peptides

Autor: Deepak Patel

Limbă:

Legare: Carte broșată

Editura: VDM Verlag Dr. Müller

Disponibilitate: La editor doar la comandă

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253.91 lei

Hydroxylysine is produced as a posttranslational modification mainly in collagens, the most abundant...

Informații despre carte

Autor

Deepak Patel

Limbă

engleză

Legare

Carte - Carte broșată

Publicat

2010

Pagini

EAN

9783639222579

Enbook ID

06830083

Editura

VDM Verlag Dr. Müller

Greutate

118

Dimensiuni

150 x 220 x 4

Descriere completă

Hydroxylysine is produced as a posttranslational modification mainly in collagens, the most abundant protein in mammals. Lysyl hydroxylase (LH) is the enzyme that catalyzes the formation of hydroxylysyl residues in collagen by hydroxylation of -X-Lys-Gly- sequences. In order to study the hydroxylation reaction catalysed by LH, we have synthesized 4 different peptides and the corresponding hydroxylated peptide using Fmoc solid-phase methodology. Peptides have been characterized by HPLC, mass spectrometry and CD spectroscopy. A new HPLC method for efficient separation of lysine- from hydroxylysine-containing peptides has been developed in both organic (1-anthroylnitrile as derivatizating reagent) and aqueous phase (dansyl chloride as derivatizating reagent). Peptides derivatization is done prior to HPLC analysis. The products (di- and tetra-substituted lysine- and hydroxylysine- containing peptides) have been fully separated by HPLC and their structure confirmed by MALDI-TOF MS analysis. Efficient separation of derivatized peptides will allow for the convenient and rapid measurement of LH activity by HPLC methods.

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Assay Development for Lysyl Hydroxylase

A new HPLC method for efficient separation of lysine- from hydroxylysine-containing peptides

Informații despre carte

Descriere completă

S-ar putea să te intereseze

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