Animal Cell Electroporation and Electrofusion Protocols

Name: Animal Cell Electroporation and Electrofusion Protocols
Brand: Humana Press Inc.
SKU: 9781489940612
Price: 297.36 RON
Availability: InStock

Autor: Jac Nickoloff

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Legare: Carte broșată

Editura: Humana Press Inc.

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297.36 lei

The ability to introduce macromolecules into animal cells, includ ing DNA, RNA, proteins, and other...

Informații despre carte

Autor

Jac Nickoloff

Limbă

engleză

Legare

Carte - Carte broșată

Publicat

2013

Pagini

370

EAN

9781489940612

ISBN

1489940618

Enbook ID

05182143

Editura

Humana Press Inc.

Greutate

572

Dimensiuni

152 x 229 x 22

Descriere completă

The ability to introduce macromolecules into animal cells, includ ing DNA, RNA, proteins, and other bioactive compounds has facili tated a broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud ies. Gene transfer technology in particular will continue to play an essential role in studies aimed at improving our understanding of the relationships between the gene structure and function, and it has impor tant practical applications in both biotechnology and biomedicine, as evidenced by the current intense interest in gene therapy. Although DNA and other macromolecules may be introduced into cells by a variety of methods, including chemical treatments and microinjection, el- troporation has proven to be simpler to perform, more efficient, and effective with a wider variety of cell types than other techniques. The early and broad success of electric field-mediated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, including RNA, enzymes, antibodies, and analytic dyes. Animal Cell Electroporation and Electrofusion Protocols begins with three chapters that describe the theoretical and practical aspects of electroporation, including a review of the commercially available instrumentation. These introductory chapters will be of particular inter est to those new to electric field technologies and to those developing protocols for as yet untested species or cell types. Nineteen chapters follow that present well-tested protocols for electroporation of proteins and DNA into insect, fish, and mammalian cells.

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Animal Cell Electroporation and Electrofusion Protocols

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