AMPK

Autor: Dietbert Neumann, Benoit Viollet

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Legare: Copertă tare

Editura: Humana Press Inc.

Disponibilitate: În depozitul extern în cantități mici

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This volume explores the latest methods used to study AMPK by computational, biochemical, biophysica...

Informații despre carte

Autor

Dietbert Neumann, Benoit Viollet

Limbă

engleză

Legare

Carte - Copertă tare

Publicat

2018

Pagini

626

EAN

9781493975976

ISBN

1493975978

Enbook ID

18257695

Editura

Humana Press Inc.

Greutate

1380

Dimensiuni

263 x 191 x 45

Descriere completă

This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to measure the effects of regulatory ligands on AMPK; use of sensors of AMPK activity; AMPK protein interaction by yeast two-hybrid; the role of AMPK in inflammation and autophagy; analyzing the AMPK function in C. elegans and mammals (with special focus on skeletal muscle, blood vessels, kidneys, pancreatic islets and hypothalamus); and human Gamma2 AMPK mutations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, AMPK: Methods and Protocols is a valuable resource for graduate students, postdoctoral researchers, and established investigators who are interested in the richly diverse AMPK field.

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