2-D Proteome Analysis Protocols

Name: 2-D Proteome Analysis Protocols
Brand: Humana Press Inc.
SKU: 9780896035249
Price: 895.27 RON
Availability: InStock

Autor: Andrew J. Link

Limbă:

Legare: Copertă tare

Editura: Humana Press Inc.

Disponibilitate: În depozitul extern în cantități mici

Expediem în 13-18 zile

895.27 lei

With the completion of sequencing projects and the advancement of a- lytical tools for protein ident...

Informații despre carte

Autor

Andrew J. Link

Limbă

engleză

Legare

Carte - Copertă tare

Publicat

1998

Pagini

601

EAN

9780896035249

ISBN

0896035247

Enbook ID

02716573

Editura

Humana Press Inc.

Greutate

1081

Dimensiuni

155 x 235 x 33

Descriere completă

With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics the study of the expressed part of the genome has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry especially in the fields of molecular biology, biochemistry, microbiology, and cell biology should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.

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