Induced Pluripotent Stem (Ips) Cells: Methods and Protocols

1. Human Induced Pluripotent Stem Cell (iPSC) Handling Protocols: Maintenance, Expansion, and Cryopreservation

Davide Marotta, Chandrika Rao, and Valentina Fossati

2. Generation of Induced Pluripotent Stem Cells from Human Bone Marrow-Derived Mesenchymal Stem Cells

3. Analysis of Clonal Composition in Human iPSC and ESC and Derived 2D and 3D Differentiated Cultures

Bernat del Olmo, Daria Merkurjev, Likun Yao, Mel·
lina Pinsach-Abuin, Ivan Garcia-Bassets, and Angels Almenar-Queralt

4. Culturing Human Pluripotent Stem Cells on Micropatterned Silicon Surfaces

Varvara Chalmantzi, Chara Simitzi, Angelos Papadopoulos, Eleni Bagli, Carol Murphy, Emmanuel Stratakis, and Theodore Fotsis

5. Porcine iPSC Generation: Testing Different Protocols to a Successful Application

Oriol Iborra-Egea, Daina Martnez-Falguera, Santiago Roura, Antoni Bayes-Genis, ngel Raya, and Carolina Glvez-Montn

6. Efficient High-Density hiPSCs Expansion in Simple Dialysis Device

Fuad Gandhi Torizal, Hyunjin Choi, Marie Shinohara, and Yasuyuki Sakai

7. Expanding the Differentiation Potential of Already-Established Pluripotent Stem Cells

Jos Gonzlez-Martnez and Marcos Malumbres

8. Generation of Quiescent Cardiac Fibroblasts Derived from Human Induced Pluripotent Stem Cells

Hao Zhang, Mengcheng Shen, and Joseph C. Wu

9. Inductive Co-Culture Differentiation of Induced Pluripotent Stem Cells into Cardiomyocytes

Axel J. Chu and Chinten James Lim

10. 3D Microwell Platform for Cardiomyocyte Differentiation of Human Pluripotent Stem Cells

Mariana A. Branco, Tiago P. Dias, Joo P. Cotovio, Carlos A.V. Rodrigues, Tiago G. Fernandes, Joaquim M.S. Cabral, and Maria Margarida Diogo

11. Scalable Generation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Sarkawt Hamad, Daniel Derichsweiler, Jrgen Hescheler, and Kurt Pfannkuche

12. Application of Human Induced Pluripotent Stem Cell Technology for Cardiovascular Regenerative Pharmacology

Qasim A. Majid, Barbara Orsolits, Lotta Pohjolainen, Zsfia Kovcs, Gbor Fldes, and Virpi Talman

13. Efficient and Safe Method of Generating Induced Pluripotent Stem Cells from Human Skin Fibroblasts and Subsequent Differentiation into Functional Cardiomyocytes

Sheeja Rajasingh, Vinoth Sigamani, Narasimman Gurusamy, and Johnson Rajasingh

14. An Optical-Flow-Based Method to Quantify Dynamic Behavior of Human Pluripotent Stem Cell-Derived Cardiomyocytes in Disease Modeling Platforms

Mohammad Izadifar, Tnde Berecz, gota Apti