Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis

In the past few years, the number of applications of tracers for invivo biomedical studies has greatly increased. New analytical toolsat the genetic and protein levels have spurred this growth, openingthe door for a deeper understanding of metabolic events. This inturn promises to yield significant advances in the understandingand treatment of human disease.

Now fully revised and expanded, Isotope Tracers in MetabolicResearch, Second Edition is the established definitive text onstable and radioactive isotope tracers. In unique, multidisciplinary fashion, it presents comprehensive coverage ofnew methodological, mathematical, and theoretical approaches.

This new Second Edition includes:

• All-new chapters on nuclear magnetic resonance, mass isotopomeranalysis, and methods of protein metabolism analysis
• A completely updated categorized list of over 750references
• Major advances in the development of mass isotopomer andpositional isotopomer techniques, noninvasive isotope techniquesfor studying metabolic pathways, hyphenated techniques, and newtracer techniques
• The latest developments in quantification of DNA synthesis andmass spectrometry spurred by genome sequencing and proteomics
• New coverage of mathematical modeling
• Expanded coverage of microdialysis probes, laboratoryprocedures, and regulatory issues related to human studies

In this complete guide to performing tracer studies, the authorssystematically cover tracer selection, modeling considerations, sample derivitization, mass spectrometry analysis, and datainterpretation. Problems and discussion questions highlight keypoints in each chapter. Isotope Tracers in MetabolicResearch, Second Edition offers students and researchers acomprehensive, practical resource for utilizing the latest tracermethodologies.